Annie FRELET-BARRAND

Chercheur CNRS

afb photo 2016
Biochimie, Biotechnologie

  • Tél. : 03 63 08 26 54
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  • Adresse :
    Institut FEMTO-ST
    15B avenue des Montboucons
    25030 Besancon Cedex
  • Lien Google Scholar

Carrière

  • Depuis Sept 2015 : Chargée de recherche CNRS (CR1), Section 20, Groupe BioMicroDevices, Département MicroNanoSciences et Systèmes (MN2S), Institut FEMTO-ST, Besançon (25).
  • Oct 2009 – Aout 2015 : Chargée de recherche CNRS (CR2 et CR1 en 2014), Section 20, Laboratoire de Stress Oxydant et Détoxication, I2BC CEA Saclay, Gif-sur-Yvette (91)
  • Sept 2006 – Sept 2009 : Stage post-doctoral, Laboratoire de Physiologie Cellulaire Végétale, CEA Grenoble (38)
  • Sept 2000 à Oct 2003 : Thèse en Ingénierie enzymatique et biochimique, Laboratoire de Génie Enzymatique et Cellulaire, UMR CNRS 6022, Université de Technologie de Compiègne (60), sous la direction du Pr Christian Bourdillon

Enseignement

  • Master 2 RHG Besançon

Publications

  • Klein M*, Perfus-Barbeoch L*, Frelet A, Gaedeke N, Reinhardt D, Mueller-Roeber B, Martinoia E and Forestier C (2003) The plant multidrug resistance ABC transporter AtMRP5 is involved in guard cell hormonal signalling and water use. Plant J. 33: 119:129.
  • Frelet A and Klein M (2006) Insight in eukaryotic ABC transporter function by mutation analysis. FEBS Lett. 580, 1064-1084.
  • Suh SJ*, Wang YF*, Frelet A*, Leonhardt N*, Klein M, Forestier C, Mueller-Roeber B, Cho MH, Martinoia E and Schroeder JI (2007) The ATP binding cassette transporter AtMRP5 modulates anion and calcium channel activities in Arabidopsis guard cells. J. Biol. Chem. 282:1916-1924.
  • Frelet-Barrand A, Kolukisaoglu HU, Plaza S, Rüffer M, Azevedo L, Hörtensteiner S, Marinova K, Weder B, Schulz B and Klein M (2008) Comparative mutant analysis of Arabidopsis ABCC-type ABC transporters: AtMRP2 Contributes to detoxification, vacuolar organic anion transport and chlorophyll degradation. Plant Cell Physiol. 49: 557-569
  • Krammer EM, Ravaud S, Dehez F, Frelet-Barrand A, Pebay-Peyroula E and Chipot C (2009). High-chloride concentrations abolish the binding of adenine nucleotides in the mitochondrial ADP/ATP carrier family. Biophys J 97:L25-27.
  • Nagy R, Grob H, Weder B, Green P, Klein M, Frelet-Barrand A, Schjoerring JK, Brearley CA and Martinoia E (2009) The Arabidopsis ATP-binding cassette protein ATMRP5/ATABCC5 is a high-affinity inositol hexakisphosphate transporter involved in guard cell signaling and phytate storage. J. Biol. Chem. 284:33614-33622.
  • Frelet-Barrand A*, Boutigny S*, Moyet L, Deniaud A, Seigneurin-Berny D, Salvi D, Bernaudat F, Richaud P, Pebay-Peyroula E, Joyard J and Rolland N (2010) Lactococcus lactis, an alternative system for functional expression of peripheral and intrinsic Arabidopsis membrane proteins. Plos One e8746
  • Frelet-Barrand A, Boutigny S, Kunji E and Rolland N (2010) Membrane protein expression in Lactococcus lactis. Methods Mol. Biol. 601:67-85.
  • Deniaud A, Bernaudat F, Frelet-Barrand A, Juillan-Binard C, Vernet T, Rolland N and Pebay-Peyroula E (2011) Expression of a chloroplast ATP/ADP transporter in E. coli membranes: Behind the Mistic strategy. Biochim Biophys Acta 1808:2059-2066.
  • Bernaudat F*, Frelet-Barrand A*, Pochon N, Dementin S, Hivin P, Boutigny S, Rioux JB, Salvi D, Seigneurin-Berny D, Richaud P, Joyard J, Pignol D, Sabaty M, Desnos T, Pebay-Peyroula E, Darrouzet E, Vernet T, Rolland N (2011). Heterologous expression of membrane proteins: choosing the appropriate host. PLoS One e29191.
  • Deniaud A, Panwar P, Frelet-Barrand A, Bernaudat F, Juillan-Binard C, Ebel C, Rolland N, Pebay-Peyroula E (2012) Oligomeric status and nucleotide binding properties of the plastid ATP/ADP transporter 1: toward a molecular understanding of the transport mechanism. PLoS One e32325.
  • Boutigny S, Sautron E, Finazzi G, Rivasseau C, Frelet-Barrand A, Pilon M, Rolland N, Seigneurin-Berny D (2014) HMA1 and PAA1, two chloroplast envelope PIB-ATPases, play distinct roles in chloroplast copper homeostasis. J Exp Bot 65:1529-1540.
  • Boutigny S, Sautron E, Frelet-Barrand A, Moyet L, Salvi D, Rolland N, Seigneurin-Berny D (2014) Functional expression of plant membrane proteins in Lactococcus lactis. Methods Mol Biol 1258:147-65.
  • Bakari S, André F, Seigneurin-Berny D, Delaforge M, Rolland N, Frelet-Barrand A (2014) Lactococcus lactis, recent developments in functional expression of membrane proteins. Chapter 5 of “Membrane Proteins Production for Structural Analysis”, edited by I. Mus−Vuteau (Springer eBook) pp 107−132.
  • Bakari S, Lembrouk M, Sourd L, Ousalem F, André F, Orlowski S, Delaforge M, Frelet-Barrand A (2016) Lactococcus lactis is an Efficient Expression System for Mammalian Membrane Proteins Involved in Liver Detoxification, CYP3A4, and MGST1. Mol Biotechnol. 58:299-310.
  • Seigneurin-Berny D, King M, Sautron E, Moyet L, Catty P, André F, Rolland N, Kunji E, Frelet-Barrand A. (2016) Membrane protein production in Lactococcus lactis for functional studies. Methods Mol Biol (In Press)

Conférences invitées

  • CNRS/GDR2478. Frelet A.Lactococcus lactis, an efficient factory for the production of recombinant membrane proteins. Grasse (06, France), 16-19 juin 2008.
  •  Séminaire IBEB/SBVME. Frelet-Barrand A. Lactococcus lactis, un système adapté à l’expression de protéines membranaires. CEA Cadarache. 26 mars 2009.
  • Congrès du GFB.Frelet-Barrand A, Boutigny S, Moyet L, Deniaud A, Seigneurin-Berny D, Salvi D, Bernaudat F, Pebay-Peyroula E, Joyard J and Rolland N. Lactococcus lactis, un système alternatif pour l’expression fonctionnelle de protéines membranaires. Saint-Pierre D’Oléron, 21 au 25 septembre 2011
  • CHI Third Annual PEGS Europe, Protein and Antibody Engineering Summit, Frelet-Barrand A. Lactococcus lactis, an Alternative System for Functional Expression of Membrane Proteins. Hannovre (Allemagne), 11−13 octobre 2011.
  • CHI Fourth Annual PEGS Europe, Protein and Antibody Engineering Summit. Frelet-Barrand A. Heterologous Expression of membrane proteins: choosing the appropriate host. Vienne (Autriche), 6-8 novembre 2012.
  • CHI Fifth Annual PEGS Europe, Protein and Antibody Engineering Summit. Frelet-Barrand A. Lactococcus lactis: alternative and emerging system for functional expression of difficult membrane proteins. Lisbon (Portugal), 6-8 novembre 2013.